Vitamin C Determination By Iodine Titration Biology Essay

Vitamin C determination By Iodine Titration Biology EssayVitamin C is as well cognize as ascorbic acidulated, it is an antioxidant that is essential for human nutrition. Antioxidants help to ruby-reduce the dam eld to the tree trunk ca utilize by toxic chemicals and pollutants. Vitamin C is a water-soluble vitamin meaning that it dissolves in water, it is essential for outgrowth and repair of all body tissues. Collagen is an primal protein that is determinationd to make skin, bulls eye tissue, tendons, ligaments and blood vessels. Vitamin C is essential to form this and is in like manner required to reanimate wounds, repair and of importtain cartilage, bones and teeth. The body does non store vitamin C and cannot make it by itself, therefore it is classic for scores of vitamin C-containing sustenances to be included in the diet. Excellent sources of vitamin C be kilobyte peppers, citrus fruits, strawberries, broccoli, papaya, watermelon and cauliflower. Vitamin C d eficiency can lead to many an(prenominal) problems in the body including dry and splitting hair, bleeding gums, easy bruising, anemia and nosebleeds with one of the most serious being scurvy. Eating a equilibrise diet is the best way to get the daily requirements of vitamin C and otherwise essential vitamins. The Food and Nutrition Board at the Institute of medical specialty recommends the following amounts of vitamin CInfants and Children0 6 months 40 milligrams/ twenty-four hours (mg/day)7 12 months 50 mg/day1 3 eld 15 mg/day4 8 years 25 mg/day9 13 years 45 mg/dayAdolescentsGirls 14 18 years 65 mg/dayBoys 14 18 years 75 mg/dayAdults workforce age 19 and older 90 mg/dayWomen age 19 year and older 75 mg/day unrivalled way to establish the amount of vitamin C in food is to workout a redox titration. The redox reaction is better than an acid-base titration since there atomic number 18 impartitional acids in a juice, but few of them interfere with the oxidization of asc orbic acid by tincture of iodine. Iodine is relatively insoluble, but this can be improved by complexing the iodine with iodide to form triiodideI2 + I- I3-Triiodide oxidizes vitamin C to form dehydroascorbic acidC6H8O6 + I3- + H2O C6H6O6 + 3I- + 2H+As long as vitamin C is present in the solution, the triiodide is converted to the iodide ion very quickly. However, when the all the vitamin C is oxidized, iodine and triiodide will be present, which react with amylum to form a blue-black complex. The blue-black colouring material is the end spot of the titration.Vitamin C Ascorbic AcidMethodThe 1% starch solution, iodine solution and vitamin C solution were made up by the technicians. The solutions were value before the noncitizen samples were titrated using the following method25.00 ml of vitamin C standard solution was added to a 125ml Erlenmeyer flask10 drops of 1% starch solution were added to the flaskThe burette was rinsed with a small volume of the iodine solution and t hen filled, the initial volume was recorded.The solution was titrated until the end point was reachedThe final volume of the iodine solution was recordedThe titration was completed doubly more.Samples of red pepper juice, apple juice and acetum were then titrated in the same way. This is an example of a titrationResultsSampleYour Hypothesis high spiritualist or low Vitamin C content?Titration 1Titration 2Titration 3Calculated Vitamin C content (g/l)Your result versus the HypothesisRed spice up juiceHigh Vitamin C content16.017.018.028.5CorrectApple succusMedium Vitamin C content4.04.04.06.8CorrectVinegarLow Vitamin C content0.40.20.40.67CorrectAnalysisThe ml of titrant used for each flask is calculated usingAverage volume = total volume occur of trialsThe amount of vitamin C in the samples are calculated usingV itamin C titrant = Titrant used in SampleStandard of Vitamin C unknownRed Pepper Juice Titrant = Used SampleStandard of Vitamin C Unknown Vitamin C 14.9ml = 17 0.025g/ml P596 = 17.0 PP= 17.0 P= 0.0285g/ml 596Litres = 0.0285 x 1000 = 28.5g/litreApple Juice Titrant = Used SampleStandard of Vitamin C Unknown Vitamin C 14.9ml = 4.0 0.025g/ml A596 = 4.0 AA= 4.0 A= 0.0067 g/ml 596Litres = 0.0067 x 1000 = 6.8g/litreVinegar Titrant = Used SampleStandard of Vitamin C Unknown Vitamin C 14.9ml = 0.4 0.025g/ml G596 = 0.4 GG= 0.4 G= 0.00067g/ml 596Litres = 0.00067 x 1000 = 0.67g/litreDiscussionOne of the main(prenominal) problems when completing this investigate is that the concentration of solution was unknown as it was nimble by the technician, the concentration was too high so had to be diluted. The supposition was correct in that the red pepper juice had the highest vitamin C content, vinegar the lowest and apple juice medium. Many safety procedures were carried out during the experiment safety glasses, gloves and goggles were worn, all bags and jackets were in the lockers and chairs were tucked under the desk. in that location was a good experience with the number of people in the root word as it gave everyone a chance to be involved with the experiment. A breeding was taken at the first point when the colour started to change and it could be seen easily when it was complete as the change happened gradually. Two examples of other food that are rich in vitamin C that were not tested are tomatoes and raspberries. Iron increases the absorption of vitamin C in the human body, iron is important as it is used by the body in the production of red blood cells, it is specifically used in the production of hemoglobin which is the main protein in red blood cells. Polyphenol oxidase, also known as tyrosinase, is an enzyme contained in fruits and vegetables such as apples, pears, potatoes, and other starchy foods. When oxygen comes in to affaire with the enzyme it causes the fruit to turn brown. Vitamin C has many functions in the body, it is essential for growth and repair of all body tissues. Collagen is an important protein that is u sed to make skin, scar tissue, tendons, ligaments and blood vessels. Vitamin C is essential to form this and is also required to cure wounds, repair and maintain cartilage, bones and teeth. Anti-oxidant vitamins are Vitamins that help the body in cleansing itself from waste and radical free agents, Free radicals are atoms or groups of atoms with an (unpaired) number of electrons and can be formed when oxygen interacts with certain molecules. As well as vitamin C, vitamins A and E are also antioxidants. Vitamin A helps form and maintain healthy teeth, skeletal and soft tissue, mucous membranes, and skin. It is also known as retinol because it produces the pigments in the retina of the eye. Vitamin E is important in the formation of red blood cells and can help the body to use vitamin K. Selenium is an essential trace mineral that works strongly with vitamin E to provide the body with powerful antioxidant protection from the free radicals that may add to the development of cancer, hea rt disease, and arthritis. Selenium protects the body from free radical pervert in two ways It is included into proteins to make selenoproteins, which are important antioxidant enzymes, and it helps the body manufacture its own natural antioxidant, glutathione.ConclusionIn conclusion, the experiment worked well as the amount of vitamin C in the samples was established and the results were hi-fi and as expected.